end-point assay: (in biomolecular screening) Kinetic assay run for ... although purists reserve the former for screening of compound libraries and the latter for more generalized cell biology experiments. enzyme [en´zīm] any protein that acts as a catalyst, increasing the rate at which a chemical reaction occurs. Transfection is employed in a variety of research fields. An assay is a breakdown of a material, examining the individual parts that make up the whole. The human body probably contains about 10,000 different enzymes. an analysis of a substance, esp. PolishPatents. Define assay. Giga-fren. At body temperature, very few biochemical reactions proceed at a significant rate without the presence of an enzyme. of an ore or drug: His find required an assay … It is a "sandwich" assay in which the proteins are captured locally as they are secreted by the plated cells, and detection is with a precipitating substrate. Bioassay definition, determination of the biological activity or potency of a substance, as a vitamin or hormone, by testing its effect on the growth of an organism. Transfection, technique used to insert foreign nucleic acid (DNA or RNA) into a cell, typically with the intention of altering cellular properties. The pull-down assay is an in vitro method used to determine a physical interaction between two or more proteins. Immunoassay definition is - a technique or test used to detect the presence or quantity of a substance (such as a protein) based on its capacity to act as an antigen. Two types of transfection, transient and stable, are used experimentally. Example sentences with "serological assay", translation memory. translation and definition "serological assay", Dictionary English-English online. Diagnosis requires virologic methods based on PCR or serologic assays using the E2 antibody. See more. The way an assay is carried out (the method of testing to determine the purity of enzymes) will influence the reported activity values. serological assay. Phenotypic screening of compounds in a multicellular humanized environment provides richer data for faster translation into the clinic. assay synonyms, assay pronunciation, assay translation, English dictionary definition of assay. ELISA is the basic assay technique, known as enzyme-linked immunosorbent assay (also referred to as EIA: Enzyme Immunoassay) that is carried out to detect and measure antibodies, hormones, peptides and proteins in the blood. ELISPOT (enzyme-linked immunospot assay) refers to ELISA-like capture and measurement of proteins secreted by cells that are plated in PVDF-membrane-backed microplate wells. The ELISA Assay Understand how an ELISA is performed, learn what serum components are measured in an ELISA, and be able to explain how an ELISA test is used to detect sero-positivity for HIV or any other pathogen. 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